Read signals in the near-Infrared spectrum

Using near IR dyes can considerably reduce background noise in protein microarrays such as nitrocellulose or PVDF.

Increased sensitivity of protein microarrays thanks to Infrared fluorescence detection: with its two sources of excitation in the near infrared spectrum (670 nm and 785nm), autofluorescence can be reduced in nitrocellulose or PVDF slides. This leads to increased sensitivity by considerably increasing the signal/background noise ratio.

Real-time autofocus is optimized for nitrocellulose or PVDF substrates: The Innoscan 710-IR scanner uses a real-time autofocus system giving a perfect homogeneity across the entire reading surface. A special configuration allows users to adapt the autofocus system to nitrocellulose or PVDF formats.

Speed of 10 to 35 lines per second means an entire slide is read in 4 minutes (10 µ)

The Innoscan 710-IR is ideal for reading slides on a nitrocellulose or PVDF support, with any near infrared dyes (670nm, 785nm):

• Protein microarrays
• ELISA arrays
• RPPA
• Enzymatic microarrays

 

Application Fields :

• Proteomic : High-throughput protein expression detection.
• Cell signal studies: profiling of proteins and protein activity tied to cellular signal pathways.

The Innoscan 710-IR is a confocal fluorescence scanner equipped with two excitation lasers (670nm and 785nm) and two photomultiplier tubes (PMT). The PMT detection system is extremely sensitive and perfectly linear across the entire detection range (0 to 100%) allowing users to adjust the PMT gain of each wavelength independently .

 

Thanks to real-time autofocus system, the effect of substrate deformation is eliminated, giving perfect homogeneity across the entire reading surface. Innopsys’ InnoScan scanners are known for their excellent photometric properties, notably in terms of sensitivity and signal to noise ratio (SNR).

 

The XDR mode (Extended Dynamic Range), provides a 6 log dynamic range maximising low signal detection whilst avoiding saturated spots.

IR detection increases sensitivity

Further Information

Resolution

3 - 40 µm / pixel

Excition Wavelengths

670 nm and 785 nm

Compatible Fluorophores

670 nm : AlexaFluor 700, Dylight 680, IRDye 680, AlexaFluor 680

785 nm : AlexaFluor 790, IRDye 800, Dy-light 800

Compatible Slides

Microscope slides of the standard norm ISO 8037-1

25-26 x 75-76 mm2 / 1” x 3” ; thickness: 0.9 - 1.2 mm

Maximum Reading Zone

22 x 74 mm²

Focus

Automatic or manual

Detection

Digital PMT and real confocal detection

PMT Gain

Linear from 0 to 100%

Reading Modes

Simultaneous or sequential

Reading Speed

10 to 35 lines/sec

Read Time

3,55 minutes for a full slide scan at a resolution of 10 µm / pixel

Dimensions

12 x 18 x 16 in

Weight

15,5 Kg (35 lbs)

Interface

Ethernet

Bar Code Reader

Yes

Image Analysis Program

Mapix

Image Format

16 and 20 bit TIFF